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The concentration of piracetam achieved in central nervous system after a typical dose of 1200 mg (about 100 M) [29] is much higher than the concentration necessary to inhibit N-type calcium channels (IC50 of piracetam in rat neurons was 3 M)

Be sure to follow the instruction guide for each weekly dose

The mixture was vortexed for 5 min at 4C and centrifuged at 15,000 rpm for 15 min at 4C

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