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The reactions range from a mild pink circle that fades in hours to angry welts that spread across the abdomen over days, causing burning, itching, and genuine discomfort

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The cell pellets were thawed and lysed in a buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 10 mM MgCl 2 , 1 mM MnCl 2 , and 100 M TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by Dounce homogenization