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However, it is important to note that erythrocytes generate ROS primarily as a result of (i) hemoglobin oxidation, which can be considered as an iron-mediated Fenton reaction, (ii) the activity of NAPDH oxidase and (iii) xanthine oxidoreductase, while in nucleated cells ROS are mainly generated in the mitochondrial electron transport chain

The accumulation of free radicals is one of the most important factors that accelerate this process

Relative mRNA expression was determined using the GoTaq 1-step real-time quantitative PCR (RT-qPCR) system (TAKARA) and qPCR using SYBR premix Ex Taq kit (TAKARA), and RT-qPCR was performed on an ABI Prism 7500 Fast Real-Time PCR System (Applied Biosystems, CA, USA)

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