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In parallel, KPV apparently lowered IL-8 output, and in immune cell cultures stimulated with TNF- it apparently preserved IB- while trimming IL-8 messenger RNA

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Then cells were blocked with 2% bovine serum albumin (BSA) in PBS, followed by incubation with primary antibodies, HSP60 and TOMM20 (detailed in Supplementary Table S3), in 2% BSA in PBS, for 1 h at room temperature

NAD+ supports fundamental energy pathways in all cells, including cancer cells, raising concern that artificially boosting NAD+ might fuel malignancy growth or interfere with certain cancer treatments