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Early studies pointed to the plasma membrane as the primary execution site, supported by findings that FSP1, as described above, exerts its antiferroptotic function through the reduction of plasma membrane ubiquinone (CoQ 10 ) 16,17

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Before You Begin: What You Need Equipment Laminar flow hood or clean bench (sterile technique recommended) Calibrated micropipettes (P200, P1000) Low-binding microcentrifuge tubes (1.5 mL) Vortex mixer Centrifuge (benchtop, capable of 10,00014,000 rpm) Ice bath or cooling block Materials Lyophilized IGF-1 LR3 (from Palmetto Peptides see IGF-1 LR3 research peptide) Reconstitution solvent (see Solvent Selection section below) Sterile water for injection (WFI) or HPLC-grade sterile water 0.22 m syringe filter (low-protein-binding membrane, e.g., PES or PVDF) Sterile syringes Parafilm or sealing film Marker for labeling Personal Protective Equipment Lab coat Nitrile gloves Safety glasses Step 1: Solvent Selection Solvent choice is the most critical variable in IGF-1 LR3 reconstitution