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Prior to puromycin selection (Day-1), plates were imaged using an Incucyte Zoom Imaging System (Essen Bioscience, USA) to confirm induction of lentiviral expression by doxycycline
TB-500 binds monomeric G-actin through the central LKKTETQ motif, sequestering actin monomers and modulating the cytoplasmic pool available for filament elongation in cell-based assays
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