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Intracellular Anabolic Signaling Modulation: Activation of IGF-1 receptorassociated pathways influences intracellular signaling networks governing protein synthesis

Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark

Atomic model refinement The initial template for the AMYR:Cagri/CagriBB complex was generated by changing the divergent resides of rAmy in the corresponding AMYR:rAmy complex (PDB: 7TYF,7TYX, 7TZF)