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Following homogenization, 120 L of tissue homogenate was incubated with 0.2 mM mito-TEMPO-H in KrebsHenseleit KHB containing 100 M in 200 L of total volume for 1 h at 37 C
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The Maillard reaction involves many chemical processes that are initiated by direct interactions between a carboxyl or hemiacetal group in a reducing sugar and an amine group in an amino acid or a peptide
consequently creating a cellular oxidative environment which triggers the oxidation of essential biomolecules like DNA, protein and lipids, leading to multiple disease conditions
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