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The cells were incubated with rabbit-anti-FLAG (1:800) (#14793S, Cell Signaling Technology) diluted in blocking buffer overnight at 4 C, washed 3 times 10 min in TBS plus 0.05% Tween-20, and subsequently incubated with secondary antibodies Donkey anti-rabbit Alexa Fluor Plus 555 (A32794 ThermoFischer) and Donkey anti-goat Alexa Fluor Plus 647 (A32849, ThermoFischer) diluted 1:500 and 10 g/mL Hoechst 33342 Solution (Thermo Scientific) (1:1000) in blocking buffer
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