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Carbohydrate utilization and digestibility by tilapia, Oreochromis niloticus O
& Morrow, T
Mice were given an oral bolus of glucose (2 g/kg) and subsequently anesthetized with 4% isoflurane
The inserts were removed, the wells were washed with phenol red-free DMEM/HAMs F-12 medium supplemented with 5% FBS and the uptake of nanocarriers in the organoids was immediately visualized by confocal laser scanning microscope (Olympus Fluoview FV1000, Olympus Life Science Europa GmbH, Germany)
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