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The beads were washed three times for 5 min each with low-salt wash buffer containing 20 mM Tris-HCl, 1% Triton X-100, 2 mM EDTA, 150 mM NaCl, and 0.1% SDS

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Research demonstrates anti-inflammatory effects in contact hypersensitivity models (skin), human keratinocyte cultures, and human bronchial epithelial cells