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Cryotherapy applied immediately after exercise or injury controls the acute inflammatory surge that could otherwise become counterproductive

Adenosine receptors and epilepsy: current evidence and future potential

New York, NY: Springer (2014)

The cells were then harvested by centrifugation at 1000 g for 3 min at room temperature and resuspended in 100 mM MES/Tris buffer, pH 6, to an OD 600 of 7.5

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