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At day 6, cells were washed in PBS (Gibco, 10010-023), collected in PBS and centrifuged for 5 min at 4 C

This paradoxical condition fulfils two fundamental tasks: (a) it provides the biosynthesis of FA important for cancer propagation, while ensuring an important source of ATP and Nicotinamide adenine dinucleotide (NADH) by the catabolism of any FA excess

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By enhancing intracellular hydroperoxides, especially hydrogen peroxide, decreasing reduced glutathione concentration, increasing cysteine oxidation, and activating protein kinases, oxidant stress can enhance the activity of transcription factors, such as the nuclear factor kappa B and activator protein-1, which are involved in the expression of cell surface adhesion molecules, growth factors, immunoreceptors, and cytokines