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Assessment of oxidant/antioxidant biomarkers The preserved testis of each rat was homogenized in cold PBS (pH 7.4), centrifuged at 4000 rpm at 4C for 15 min, and the obtained supernatants were used for investigation of MDA level, GSH content, and CAT activity, following the manufacturer's instructions of the commercial kits and according to the methods described by Ohkawa et al (1979), Beutler et al
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