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Furthermore, GLP-1 receptor agonists that are renally eliminated, such as lixisenatide and exenatide, are contraindicated in severe renal disease, which is more prevalent in this population

Small interfering RNA construction Using previously reported rational criteria 31,32 , we designed the following target sequences to get gene expression-specific knockdown by short interfering RNA (siRNA)

We plotted the continuous change in expression of and cell identity markers ARX, DNA methyltransferase 1 (DNMT1 ), MAFA , and PDX1 in the and cell subclusters (Figure 3E)