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Beelman RB, Kalaras MD, Richie JP (2019) Micronutrients and bioactive compounds in mushrooms: a recipe for healthy aging
GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating
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