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Nuclear receptor agonists, particularly FXR modulators, have demonstrated efficacy in attenuating bile acid cytotoxicity, enhancing hepatobiliary excretion, and mitigating bilirubin accumulation [54]

The most pronounced differences were observed across gastrointestinal and metabolic events, including nausea (9,651 vs 38,030), vomiting (4,383 vs 17,062), pancreatitis (1,389 vs 6,655), and hypoglycaemia (773 vs 1,778), all substantially lower with combination therapy (Table 1)

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After anesthetization with 10% chloral hydrate (v/w), the animals were fixed carefully on a stereotactic instrument