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10.1016/j.arr.2021.101536 25 PaiV

Wear PPE including gloves, lab coat, and protective eyewear Handle compounds within approved laboratory workspaces Avoid inhalation, ingestion, or direct contact Dispose of materials according to institutional chemical waste procedures Maintain proper labeling and documentation for stored research compounds These practices support safe and compliant laboratory operations

Methods Complex production and purification Mammalian expression vectors for Fc-IgG1 with A330C mutation and FcRIIIa (V158 allotype) with I106C mutation were prepared separately and individually or co-transfected to ExpiCHO cells (ThermoFisher A29133) according to the manufacturers instructions

If you don't get enough iron or folic acid from your diet, you can take supplements