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These groups included: a high-fat diet group (HF), a high-fat diet with Melanotan-II treatment group (HF + MT-II), a control diet group (C), and a control diet with Melanotan-II treatment group (C + MT-II)

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We collected the cultures, estimated the growth OD 600 , centrifuged the cultures at 3500 g for 1 min, and collected the supernatant for measuring d 9 -TMA, d 9 -BB, and d 9 -carnitine using LC-MS